CRISPRdirect Package Version
CRISPRdirect Package Version
Highly-Specific Guide RNA Design Tool
– Now Available Offline!
Overview
The genome editing technology CRISPR-Cas9 is widely used for various applications. In CRISPR-Cas9, binding of the guide RNA to the target sequence induces DNA cleavage by the Cas9 nuclease. However, this process can also cause cleavage of similar sequences and introduce mutations (off-target effects). Mutations in non-target genes may cause unpredictable adverse effects. Therefore, in drug development and similar fields, selecting guide RNAs with minimal off-target effects is a critical step.
CRISPRdirect is a tool that enables the simple design of highly specific guide RNAs by utilizing the high-speed sequence search tool GGGenome (https://gggenome.retrieva.jp/). It supports the genomes of numerous experimental animals, plants, and crops, and provides an API for easy integration with other databases and software. The public version developed by DBCLS and NBDC is available online.
This packaged version of CRISPRdirect includes all the features and gene sequence databases of the public version, plus enables offline use for confidential projects. The entire software suite is packaged on a USB drive, eliminating the need for external connections and allowing for the concealment of search sequences. Furthermore, it is possible to add proprietary gene sequence databases.
CRISPRdirect is a tool that enables the simple design of highly specific guide RNAs by utilizing the high-speed sequence search tool GGGenome (https://gggenome.retrieva.jp/). It supports the genomes of numerous experimental animals, plants, and crops, and provides an API for easy integration with other databases and software. The public version developed by DBCLS and NBDC is available online.
This packaged version of CRISPRdirect includes all the features and gene sequence databases of the public version, plus enables offline use for confidential projects. The entire software suite is packaged on a USB drive, eliminating the need for external connections and allowing for the concealment of search sequences. Furthermore, it is possible to add proprietary gene sequence databases.
Feature
1. Designs and selects highly specific guide RNAs
CRISPRdirect can design highly specific guide RNAs (gRNAs) for CRISPR-Cas9-based genome editing across various species.
2. Capable of searching for potential off-target sites of guide RNA
The databases available for guide RNA design support over 350 species [see this link for all database list]
3. Ability to add proprietary databases
You can add and use databases owned by your organization for guide RNA design.
CRISPRdirect can design highly specific guide RNAs (gRNAs) for CRISPR-Cas9-based genome editing across various species.
2. Capable of searching for potential off-target sites of guide RNA
The databases available for guide RNA design support over 350 species [see this link for all database list]
3. Ability to add proprietary databases
You can add and use databases owned by your organization for guide RNA design.
List of bundled databases
CRISPRdirect Basic Pack
Bundled Genome Data (9 Species, 12 Databases)
・ Human GRCh38/hg38 , hg19
・ House Mouse GRCm38/mm10
・ Crab-eating macaque macFas5 , macFasRKS1912
・ Marmoset WUGSC 3.2/calJac3 , calJacRKC1912
・ Macaca mulatta BCM Mmul_8.0.1/rheMac8
・ Rat RGSC 6.0/rn6
・ Rabbit Broad/oryCun2
・ Porcine SGSC Sscrofa11.1/susScr11
・ Canine Broad CanFam3.1/CanFam3
Options
・ Optional Databases: You can add databases available for web-based GGGenome published by DBCLS.
・ Custom Databases: You can add your own nucleotide sequence databases.
Bundled Genome Data (9 Species, 12 Databases)
・ Human GRCh38/hg38 , hg19
・ House Mouse GRCm38/mm10
・ Crab-eating macaque macFas5 , macFasRKS1912
・ Marmoset WUGSC 3.2/calJac3 , calJacRKC1912
・ Macaca mulatta BCM Mmul_8.0.1/rheMac8
・ Rat RGSC 6.0/rn6
・ Rabbit Broad/oryCun2
・ Porcine SGSC Sscrofa11.1/susScr11
・ Canine Broad CanFam3.1/CanFam3
Options
・ Optional Databases: You can add databases available for web-based GGGenome published by DBCLS.
・ Custom Databases: You can add your own nucleotide sequence databases.
System Requirements
✓ This product is compatible with 64-bit versions of Windows.
✓ Windows 11 Pro version 21H2 or later, or Enterprise/Education version 21H2 or later
✓ Windows 10 Pro 21H1 or later, or Enterprise/Education 20H2 or later
✓ Hyper-V must be enabled (may be mutually exclusive with virtualization software like VMware)
✓ Memory: 8GB or more (At least 4GB must be allocated to Docker for Windows)
✓ Windows 11 Pro version 21H2 or later, or Enterprise/Education version 21H2 or later
✓ Windows 10 Pro 21H1 or later, or Enterprise/Education 20H2 or later
✓ Hyper-V must be enabled (may be mutually exclusive with virtualization software like VMware)
✓ Memory: 8GB or more (At least 4GB must be allocated to Docker for Windows)
